A characteristic of this symphony is the five-voice fugato (representing the five major themes) at the end of the fourth movement. But there are fugal sections throughout the movement either by developing one specific theme or by combining two or more themes together, as seen in the interplay between the woodwinds. The main theme consists of four notes:
Image:MozartJupiterFinaleTheme.PNG
Image:MozartJupiterFinaleTheme.PNG

Four additional themes are heard in the "Jupiter's" finale, which is in sonata form, and all five motifs are combined in the fugal coda. In a 1906 article about the Jupiter Symphony, Sir George Grove wrote that "it is for the finale that Mozart has reserved all the resources of his science, and all the power, which no one seems to have possessed to the same degree with himself, of concealing that science, and making it the vehicle for music as pleasing as it is learned. Nowhere has he achieved more." Of the piece as a whole, he wrote that "It is the greatest orchestral work of the world which preceded the French Revolution."
Scholars are certain Mozart studied Michael Haydn's Symphony No. 28 in C major, which also has a fugato in its finale. Charles Sherman speculates that Mozart also studied Haydn's Symphony No. 39 in C major since he "often requested his father Leopold to send him the latest fugue that Haydn had written." The Haydn No. 39, written only a few weeks before Mozart's, also has a fugato in the finale, the theme of which begins with two whole notes. Sherman has pointed out other similarities between the two almost perfectly contemporaneous works.

Score for the movement